4KHA

Structural basis of histone H2A-H2B recognition by the essential chaperone FACT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.872600
Spacegroup name	P 43 21 2
Unit cell lengths	108.421, 108.421, 117.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.699 - 2.350
R-factor	0.1927
Rwork	0.191
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP (Phaser)
Refinement software	PHENIX (Refine)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.078	0.011
Number of reflections	31867
<I/σ(I)>	17.6	2.3
Completeness [%]	99.9	100
Redundancy	8.8	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	manual setup 1ul protein (15 mg / ml) plus 1 ul crystallization buffer (7.25% [vol/vol] PEG8000, 0.2 M MgCl2, 0.1 M Tris pH 7.8), VAPOR DIFFUSION, HANGING DROP, temperature 277K