4KGC
Nucleosome Core Particle Containing (ETA6-P-CYMENE)-(1, 2-ETHYLENEDIAMINE)-RUTHENIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2011-07-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.50 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.620, 109.710, 181.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.600 - 2.690 |
R-factor | 0.24835 |
Rwork | 0.248 |
R-free | 0.28234 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.432 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.600 | 2.840 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 55781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40 mM MnCl2, 30 mM KCl, 20 mM K-Cacodylate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |