4KG8
Crystal structure of light mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.644, 46.911, 70.400 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.21481 |
| Rwork | 0.211 |
| R-free | 0.28608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4en0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.885 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.079 | 0.448 |
| Number of reflections | 18578 | |
| <I/σ(I)> | 24.7 | 4.694 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 290 | 0.2M AMMONIUM CITRATE DIBASIC, 20% (W/V) PEG 3350, PH 5.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
