4KFC

Crystal structure of a hyperactive mutant of response regulator KdpE complexed to its promoter DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 43 21 2
Unit cell lengths	132.770, 132.770, 134.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.320 - 2.530
R-factor	0.208
Rwork	0.204
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ZH4 and 3ZQ7
RMSD bond length	0.004
RMSD bond angle	0.928
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1352))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.430	2.620
High resolution limit [Å]	2.530	2.530
Rmerge	0.110	0.490
Number of reflections	40336
<I/σ(I)>	13.48	4.4
Completeness [%]	100.0	100
Redundancy	12	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	3-5% PEG 4K, 0.1 M sodium acetate buffer (pH 5.5), 0.1 M MgCl2, 2mM L-proline, VAPOR DIFFUSION, HANGING DROP, temperature 293K