4KBQ

Structure of the CHIP-TPR domain in complex with the Hsc70 Lid-Tail domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-17
Detector	NOIR-1
Spacegroup name	P 61 2 2
Unit cell lengths	78.500, 78.500, 424.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.746 - 2.910
R-factor	0.2274
Rwork	0.224
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2C2L 3lof
RMSD bond length	0.010
RMSD bond angle	1.251
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHENIX ((1.8.1_1168))
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.750	3.010
High resolution limit [Å]	2.910	2.910
Rmerge		0.639
Number of reflections	17188
<I/σ(I)>		4.2
Completeness [%]	97.8	76.7
Redundancy		12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.7M ammonium citrate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K