4KAS
Crystal structure of FDTS from T. maritima mutant (H53D) with FAD and dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.370, 116.670, 141.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.290 - 1.850 |
| R-factor | 0.17186 |
| Rwork | 0.170 |
| R-free | 0.21139 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB code: 1O26 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.756 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.033 | 0.568 |
| Number of reflections | 72637 | |
| <I/σ(I)> | 28.6 | 1.97 |
| Completeness [%] | 93.4 | 62.9 |
| Redundancy | 3.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 - 60% PEG200, 0.1Tris.HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
