4K96

Structure of Binary Complex of cGAS with Bound dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	200
Detector technology	CCD
Collection date	2013-01-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	181.910, 93.801, 75.509
Unit cell angles	90.00, 97.72, 90.00

Refinement procedure

Resolution	45.066 - 2.084
R-factor	0.2023
Rwork	0.202
R-free	0.22630
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.084	2.084
Number of reflections	74352
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	0.1 M MES, 8% MPD, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K