4K8X

Binary complex of 9N DNA polymerase in the replicative state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.97793
Spacegroup name	P 21 21 2
Unit cell lengths	112.215, 142.616, 66.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.712 - 2.280
R-factor	0.1976
Rwork	0.195
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qht
RMSD bond length	0.008
RMSD bond angle	1.157
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1345))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.712	2.420
High resolution limit [Å]	2.280	2.280
Number of reflections	49600
<I/σ(I)>	9.07	0.82
Completeness [%]	99.9	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Lithium chloride, 2.2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K