4K81
Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.727, 115.593, 103.107 |
| Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.22149 |
| Rwork | 0.219 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3HK0 (CHAINS A C E G) AND 1LFD (CHAINS B D F H) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.058 |
| Number of reflections | 71989 |
| <I/σ(I)> | 23.8 |
| Completeness [%] | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 315 | 14% (w/v) PEG 3350, 100 mM MES, pH 5.9, 200 mM MgCl2, 2% glycerol, and 3% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 315K |
