4K6M

Crystal Structure of the full-length Japanese encephalitis virus NS5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979305
Spacegroup name	H 3
Unit cell lengths	272.293, 272.293, 177.248
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.219 - 2.600
R-factor	0.198
Rwork	0.196
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2px2 2j7u
RMSD bond length	0.008
RMSD bond angle	1.164
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	148758
Completeness [%]	98.7	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	0.085M trisodium citrate pH 5.6, 0.17M potassium/sodium tartrate, 1.7M ammonium sulfate, 15%(v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K