4K17
Crystal Structure of mouse CARMIL residues 1-668
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9784 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.168, 67.986, 212.151 |
| Unit cell angles | 92.73, 96.95, 110.15 |
Refinement procedure
| Resolution | 49.759 - 2.895 |
| Rwork | 0.215 |
| R-free | 0.25890 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.895 | 2.895 |
| Rmerge | 0.124 | 0.825 |
| Number of reflections | 63752 | |
| <I/σ(I)> | 13.8 | 1.8 |
| Completeness [%] | 98.0 | 84.4 |
| Redundancy | 8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 16 % PEG3350, 130 mM lithium sulfate, 0.25 % w/v salicylamide, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
