4JXM

Crystal structure of RRP9 WD40 repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	P 43 21 2
Unit cell lengths	63.746, 63.746, 167.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.110 - 1.920
R-factor	0.1668
Rwork	0.165
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3psl
RMSD bond length	0.014
RMSD bond angle	1.416
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.920	5.210	1.920
Rmerge	0.184	0.071	0.906
Number of reflections	27436
<I/σ(I)>	4.7
Completeness [%]	100.0	99.5	100
Redundancy	15.4	13.5	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	25% PEG3350, 0.2 M ammonium acetate, 0.1 M HEPES sodium, pH 7.5, VAPOR DIFFUSION, temperature 291K