4JUQ

Pseudomonas aeruginosa MetAP T2N mutant, in Mn form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	80.593, 105.599, 133.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.870 - 2.200
R-factor	0.1812
Rwork	0.179
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.762
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	36.870
High resolution limit [Å]	2.200
Number of reflections	58806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop vapor diffusion	7.5		100 mM HEPES, 70% MPD, pH 7.5, hanging drop vapor diffusion