4JSV
mTOR kinase structure, mechanism and regulation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 139.400, 163.200, 207.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.500 |
| R-factor | 0.2314 |
| Rwork | 0.231 |
| R-free | 0.25080 |
| Structure solution method | MIR |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.156 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 7.540 | 3.500 |
| Rmerge | 0.185 | 0.048 | 0.665 |
| Number of reflections | 57899 | ||
| <I/σ(I)> | 3.8 | ||
| Completeness [%] | 94.6 | 95.2 | 83.6 |
| Redundancy | 3.6 | 4.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 8.5 | 277 | PEG 8000, NaCl, pH 8.5, hanging drop vapor diffusion, temperature 277K |
