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4JS4

Crystal structure of E. coli Exonuclease I in complex with a dA16 oligonucleotide

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 31-ID
Synchrotron siteAPS
Beamline31-ID
Temperature [K]100
Detector technologyCCD
Collection date2012-11-16
DetectorRAYONIX MX225HE
Wavelength(s)0.97931
Spacegroup nameP 43
Unit cell lengths91.413, 91.413, 162.518
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.600 - 3.100
R-factor0.22655
Rwork0.225
R-free0.25665
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1fxx
RMSD bond length0.004
RMSD bond angle0.714
Data reduction softwareMOSFLM
Data scaling softwareXDS
Phasing softwareAMoRE
Refinement softwareREFMAC (5.7.0029)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.6003.270
High resolution limit [Å]3.1003.100
Rmerge0.0660.813
Number of reflections22917
<I/σ(I)>22.42.9
Completeness [%]99.9100
Redundancy7.67.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82985% 2-propanol, 25% glycerol, 1.2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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