4JS1
crystal structure of human Beta-galactoside alpha-2,6-sialyltransferase 1 in complex with cytidine and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 65.292, 65.292, 162.232 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.387 - 2.090 |
| R-factor | 0.1953 |
| Rwork | 0.193 |
| R-free | 0.22820 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: dev_1327)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 22378 | |
| <I/σ(I)> | 13.5 | 1.3 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 9.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 294 | 0.1M MES/NaOH, pH 5.8, 20% PEG 2000 MME, 0.01M CaCl2, 0.01M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
