4JRP
Structure of E. coli Exonuclease I in complex with a 5cy-dT13 oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-05 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 |
Unit cell lengths | 91.300, 91.300, 159.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.580 - 1.950 |
R-factor | 0.21543 |
Rwork | 0.213 |
R-free | 0.25909 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fxx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.302 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.600 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.078 | 0.363 |
Number of reflections | 89174 | |
<I/σ(I)> | 8.4 | 2 |
Completeness [%] | 99.2 | 93.9 |
Redundancy | 3 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5 % 2-propanol, 25% glycerol, 1.2 M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |