4JRB
Structure of Cockroach Allergen Bla g 1 Tandem Repeat as a EGFP fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-BM-B |
Synchrotron site | APS |
Beamline | 23-BM-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.768, 141.920, 93.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.188 - 2.414 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ai4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.080 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.089 | 0.458 |
<I/σ(I)> | 14.9 | 2.5 |
Completeness [%] | 99.3 | 94 |
Redundancy | 7.1 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100mM Tris, 10% propanol, 20% PEG4K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |