4JPZ

Voltage-gated sodium channel 1.2 C-terminal domain in complex with FGF13U and Ca2+/calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	153.133, 86.106, 109.399
Unit cell angles	90.00, 100.90, 90.00

Refinement procedure

Resolution	48.230 - 3.020
R-factor	0.2145
Rwork	0.212
R-free	0.24580
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.170
High resolution limit [Å]	3.020	7.440	3.020
Number of reflections	53782
Completeness [%]	99.8	99.9	77.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	290	14% pEG3350, 300 mM sodium acetate,50 mM Tris pH 7.5, and 2 mM CaCl2, EVAPORATION, temperature 290K