4JPH

Crystal structure of Protein Related to DAN and Cerberus (PRDC)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	CCD
Collection date	2011-07-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9574
Spacegroup name	P 1 21 1
Unit cell lengths	73.312, 65.558, 85.077
Unit cell angles	90.00, 105.50, 90.00

Refinement procedure

Resolution	17.600 - 2.250
R-factor	0.2034
Rwork	0.202
R-free	0.22240
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.600
High resolution limit [Å]	2.250	2.250
Number of reflections	36786
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	PEG3350, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K