4JKB
Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.355, 78.159, 93.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.040 - 1.300 |
| R-factor | 0.1207 |
| Rwork | 0.119 |
| R-free | 0.14530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3enb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.442 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.6.0117) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 2.800 | 1.300 |
| Rmerge | 0.041 | 0.027 | 0.568 |
| Number of reflections | 135702 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 99.4 | 94.5 | 100 |
| Redundancy | 5.5 | 5.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K |
