4JK9
Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.228, 78.291, 94.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.270 - 1.500 |
R-factor | 0.1661 |
Rwork | 0.164 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3enb |
RMSD bond length | 0.022 |
RMSD bond angle | 1.814 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.2.0005) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.067 | 0.040 | 0.447 |
Number of reflections | 80130 | ||
<I/σ(I)> | 14.9 | ||
Completeness [%] | 88.1 | 61.6 | 96.3 |
Redundancy | 3.6 | 3.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K |