4JJZ
Crystal Structure of N10-Formyltetrahydrofolate Synthetase with ADP and Formylphosphate
Replaces: 3RBOExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-11 |
| Detector | SBC-1 |
| Wavelength(s) | 1.59 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.170, 212.976, 53.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.810 - 2.500 |
| R-factor | 0.18459 |
| Rwork | 0.181 |
| R-free | 0.25071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.709 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.590 | 2.500 |
| Rmerge | 0.085 | ||
| Number of reflections | 37221 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 91.5 | 78.9 | 64.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 278 | 75 mM Potassium Maleate Buffer pH 7.6, 1 mM dithiothreitol, 40% Ammonium Sulfate, 2% PEG 1000, vapor diffusion, hanging drop, temperature 278K |
