4JJR
A P21 crystal form of mammalian casein kinase 1 delta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.145, 102.251, 64.593 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 40.665 - 2.408 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3UZP CHAIN A |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.962 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.0) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 5.490 | 2.400 |
| Rmerge | 0.094 | 0.044 | 0.681 |
| Number of reflections | 25594 | ||
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 7.7 | 7.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | 100 mM succinic acid, 15% PEG 3350, pH 7.0, hanging drop, temperature 298K |
