4JIO
Bro1 V domain and ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-04 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.9788915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.430, 92.320, 229.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.488 - 3.600 |
| R-factor | 0.3716 |
| Rwork | 0.366 |
| R-free | 0.42110 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.665 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.490 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.084 | 0.611 |
| Number of reflections | 17664 | |
| <I/σ(I)> | 6.6 | 1.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.14 | 7.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M bis-Tris propane pH 7, 0.2-0.4 M sodium malonate, 15-20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
