4JI1

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	403.171, 403.171, 173.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.647 - 3.144
R-factor	0.1667
Rwork	0.165
R-free	0.20700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2vqe
RMSD bond length	0.007
RMSD bond angle	1.212
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.250
High resolution limit [Å]	3.140	6.750	3.140
Rmerge	0.069	0.021	0.627
Number of reflections	241842
<I/σ(I)>	13.1
Completeness [%]	99.0	95.4	97.7
Redundancy	3.4	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K