4JHD
Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 195 |
| Detector technology | CCD |
| Collection date | 2012-06-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.450, 99.800, 118.270 |
| Unit cell angles | 65.41, 90.03, 77.77 |
Refinement procedure
| Resolution | 45.020 - 2.910 |
| R-factor | 0.20317 |
| Rwork | 0.200 |
| R-free | 0.25512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.021 | 45.026 | 2.970 |
| High resolution limit [Å] | 2.910 | 7.474 | 2.910 |
| Number of reflections | 46301 | ||
| Completeness [%] | 97.7 | 98 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 10% PEG3350, 0.18M NaCl, 0.1M PIPES, protein:mother liquor = 2:1, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
