4JFN

Crystal structure of the N-terminal, growth factor-like domain of the amyloid precursor protein bound to copper

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-02
Detector	MAR CCD 165 mm
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	34.050, 49.070, 66.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.276 - 1.750
R-factor	0.1831
Rwork	0.181
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mwp
RMSD bond length	0.007
RMSD bond angle	1.156
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.280	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	11659
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	6% (w/v) PEG 3350, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K