4JBW
Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.529, 208.448, 347.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.975 - 3.913 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.28120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3g 3fh6 and 3PV0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.060 |
| High resolution limit [Å] | 3.913 | 3.913 |
| Number of reflections | 59277 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 95.0 | 96.5 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 13% PEG monomethylether 2000, 100mM Sodium cacodylate pH 5.6, 100mM Non Detergent Sulfobetaine (NDSB-256), VAPOR DIFFUSION, SITTING DROP, temperature 295K |
