4JBW

Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	94.529, 208.448, 347.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.975 - 3.913
R-factor	0.2288
Rwork	0.226
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f3g 3fh6 and 3PV0
RMSD bond length	0.002
RMSD bond angle	0.646
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	4.060
High resolution limit [Å]	3.913	3.913
Number of reflections	59277
<I/σ(I)>	13.8
Completeness [%]	95.0	96.5
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	295	13% PEG monomethylether 2000, 100mM Sodium cacodylate pH 5.6, 100mM Non Detergent Sulfobetaine (NDSB-256), VAPOR DIFFUSION, SITTING DROP, temperature 295K