4JAL
Crystal structure of tRNA (Um34/Cm34) methyltransferase TrmL from Escherichia coli with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.276, 66.291, 49.768 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 49.120 - 2.000 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jak |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 19381 | |
| Completeness [%] | 98.4 | 86.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% PEG 4000, 5% glycerol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
