4J9L
Human DNA polymerase eta-DNA ternary complex: misincorporation G opposite T after a C at the primer 3' end (CA/G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-01-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.430, 98.430, 81.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.414 - 1.850 |
| R-factor | 0.1751 |
| Rwork | 0.172 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mr2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 | 1.900 |
| High resolution limit [Å] | 1.850 | 2.070 | 1.850 |
| Rmerge | 0.101 | 0.349 | 0.803 |
| Number of reflections | 38030 | ||
| <I/σ(I)> | 14.61 | 5.33 | 2.01 |
| Completeness [%] | 98.6 | 98.2 | 96.8 |
| Redundancy | 3.8 | 3.85 | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG2K-MME, KCl, MES, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
