4J8S

Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-21
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 3
Unit cell lengths	85.060, 85.060, 85.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.070 - 1.550
R-factor	0.1676
Rwork	0.167
R-free	0.18820
Structure solution method	Sulfur SAD
RMSD bond length	0.012
RMSD bond angle	1.303
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: dev_828))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.070	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	29686
<I/σ(I)>	28.8	2.4
Completeness [%]	98.8	90.2
Redundancy	22	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2 M MgCl2, 0.1 M Bis-Tris pH 5.5, and 25 % PEG3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293K