4J8N
Aurora A Kinase Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0088 |
| Spacegroup name | P 31 |
| Unit cell lengths | 83.783, 83.783, 171.777 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.949 - 3.135 |
| R-factor | 0.1884 |
| Rwork | 0.184 |
| R-free | 0.23360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.886 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.180 |
| High resolution limit [Å] | 3.130 | 8.480 | 3.130 |
| Rmerge | 0.059 | 0.030 | 0.404 |
| Number of reflections | 23697 | ||
| <I/σ(I)> | 18.4 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 6.2 | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging drop vapor diffusion | 7 | 298 | 20 mg/ml Aurora A protein, 10% Tacsimate, 20% (w/v) PEG3350, pH 7.0, Hanging drop vapor diffusion, temperature 298K |
