4J8F

Crystal structure of a fusion protein containing the NBD of Hsp70 and the middle domain of Hip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 41
Unit cell lengths	65.596, 65.596, 141.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.197
Rwork	0.194
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.243
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.596	48.098	2.790
High resolution limit [Å]	2.650	8.370	2.650
Rmerge		0.025	0.491
Total number of observations		2770	12853
Number of reflections	17407
<I/σ(I)>	16.1	23.2	1.6
Completeness [%]	99.9	98.7	99.8
Redundancy	5.1	5	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	17 % PEG-3350 and 0.2 M NH4I, pH 7.4, vapor diffusion, hanging drop, temperature 291K