4J8A
Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.500, 58.700, 91.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.790 - 1.260 |
| R-factor | 0.1305 |
| Rwork | 0.129 |
| R-free | 0.16460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.586 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.500 |
| High resolution limit [Å] | 1.260 |
| Rmerge | 0.032 |
| Number of reflections | 67853 |
| <I/σ(I)> | 16 |
| Completeness [%] | 99.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 278 | 10 mg/mL protein and 100 mM Tris-HCl, pH 8.3, 2 M (NH4)2SO4, 200+200 nanoL drop against 60 microL reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
