4J4J
Crystal structure of the APOBEC3F Vif binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 164.046, 164.046, 135.315 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.490 - 3.100 |
R-factor | 0.2406 |
Rwork | 0.236 |
R-free | 0.27690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.495 |
Data reduction software | d*TREK (9.9.9.1D) |
Data scaling software | d*TREK (9.9.9.1D) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.490 | 43.490 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.100 | 0.046 | 0.838 |
Total number of observations | 19386 | 19991 | |
Number of reflections | 18499 | ||
<I/σ(I)> | 7.2 | 26.8 | 1.3 |
Completeness [%] | 98.5 | 97.2 | 100 |
Redundancy | 9.6 | 9.11 | 10.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 18% (w/v) PEG 8000, 0.1 M CHES pH 9.0, 25% (w/v) glucose, VAPOR DIFFUSION, HANGING DROP, temperature 298K |