4J34
Crystal Structure of kynurenine 3-monooxygenase - truncated at position 394 plus HIS tag cleaved.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.070, 98.780, 86.300 |
Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
Resolution | 56.800 - 2.030 |
R-factor | 0.2032 |
Rwork | 0.201 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 56.800 |
High resolution limit [Å] | 2.000 |
Number of reflections | 60736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Crystals were grown by mixing 200 nl of protein (buffer A) with 200 nl of a reservoir solution containing 0.1 M imidazole, pH 7.8, and 11 % w/v polyethylene glycol 8K , VAPOR DIFFUSION, SITTING DROP, temperature 277K |