4J2X
CSL (RBP-Jk) with corepressor KyoT2 bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.157, 97.293, 144.102 |
Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
Resolution | 40.300 - 2.850 |
R-factor | 0.1905 |
Rwork | 0.188 |
R-free | 0.23080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iag |
RMSD bond length | 0.010 |
RMSD bond angle | 1.290 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.300 | 2.930 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.053 | |
Number of reflections | 37773 | |
<I/σ(I)> | 13.9 | 1.82 |
Completeness [%] | 95.9 | 93.96 |
Redundancy | 5.6 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.1 | 277 | 21% PEG3350, 0.1 M HEPES, pH 7.1, 0.25 M ammonium acetate, MICROBATCH, temperature 277K |