4IXH
Crystal Structure of the Catalytic Domain of the Inosine Monophosphate Dehydrogenase from Cryptosporidium parvum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-14 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.185, 92.016, 92.139 |
Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
Resolution | 35.521 - 2.105 |
R-factor | 0.1641 |
Rwork | 0.162 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 3FFS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 83034 | |
<I/σ(I)> | 8.2 | 2.1 |
Completeness [%] | 99.2 | 96.5 |
Redundancy | 4.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 289 | 0.1 M succinic acid pH 7.0, 15 %(w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |