4IWO

Crystal structure and mechanism of activation of TBK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2011-04-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.28
Spacegroup name	P 32 2 1
Unit cell lengths	136.600, 136.600, 87.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.984 - 2.610
R-factor	0.2457
Rwork	0.245
R-free	0.26290
Structure solution method	MIR
RMSD bond length	0.003
RMSD bond angle	0.578
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	28196
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	5% PEG6000, pH 7.5, vapor diffusion, hanging drop, temperature 294K