4IUM

Equine arteritis virus papain-like protease 2 (PLP2) covalently bound to ubiquitin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-29
Detector	RAYONIX MX-300
Wavelength(s)	1.28243, 1.28289, 1.27347
Spacegroup name	P 21 21 21
Unit cell lengths	38.320, 62.040, 84.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.020 - 1.450
R-factor	0.1612
Rwork	0.160
R-free	0.17810
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.413
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.7.1_743)
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.110	1.530
High resolution limit [Å]	1.450	1.450
Rmerge	0.049	0.205
Number of reflections	36344
<I/σ(I)>		7.1
Completeness [%]	99.4	96.2
Redundancy	8.7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	100 mM MES, pH 6.2, 18% PEG20000, VAPOR DIFFUSION, HANGING DROP, temperature 293K