4IUA
Crystal Structure of the NK2 Fragment (31-290) of the mouse Hepatocyte Growth Factor/Scatter Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.620, 127.328, 129.600 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 29.775 - 3.050 |
| R-factor | 0.1829 |
| Rwork | 0.176 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hn4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 6.550 | 3.050 |
| Rmerge | 0.131 | 0.064 | 0.706 |
| Number of reflections | 57775 | ||
| <I/σ(I)> | 13.6 | 24.5 | 2.2 |
| Completeness [%] | 99.9 | 99.9 | 100 |
| Redundancy | 5.8 | 5.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200 mM ammonium sulfate, 18% PEG 4000, 200 mM HEPES pH 7.0, 5% 2-METHYL-2,4-PENTANEDIOL, 0.5 mM beta-octyl glucoside, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
