4IUA

Crystal Structure of the NK2 Fragment (31-290) of the mouse Hepatocyte Growth Factor/Scatter Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.1271
Spacegroup name	P 1 21 1
Unit cell lengths	92.620, 127.328, 129.600
Unit cell angles	90.00, 90.25, 90.00

Refinement procedure

Resolution	29.775 - 3.050
R-factor	0.1829
Rwork	0.176
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hn4
RMSD bond length	0.009
RMSD bond angle	1.266
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.160
High resolution limit [Å]	3.050	6.550	3.050
Rmerge	0.131	0.064	0.706
Number of reflections	57775
<I/σ(I)>	13.6	24.5	2.2
Completeness [%]	99.9	99.9	100
Redundancy	5.8	5.4	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	200 mM ammonium sulfate, 18% PEG 4000, 200 mM HEPES pH 7.0, 5% 2-METHYL-2,4-PENTANEDIOL, 0.5 mM beta-octyl glucoside, VAPOR DIFFUSION, HANGING DROP, temperature 298K