4IS9

Crystal Structure of the Escherichia coli LpxC/L-161,240 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	117.440, 117.440, 253.661
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.916 - 2.130
R-factor	0.2173
Rwork	0.216
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.705
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.170
High resolution limit [Å]	2.130	5.780	2.130
Rmerge	0.086	0.057	0.603
Number of reflections	37998
<I/σ(I)>	9.7
Completeness [%]	100.0	99.5	100
Redundancy	13.4	12.7	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1