4IS0
Structural Insights into Omega-Class Glutathione Transferases: A Snapshot of Enzyme Reduction and Identification of the Non-Catalytic Ligandin Site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL4-2 |
| Synchrotron site | SSRL |
| Beamline | BL4-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.034375 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.600, 57.600, 140.176 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.641 - 1.721 |
| R-factor | 0.1552 |
| Rwork | 0.152 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eem |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.424 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.640 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.081 | 0.609 |
| Number of reflections | 29422 | |
| <I/σ(I)> | 19.62 | 2.3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 16.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.25 | 277 | 2.2 M ammonium sulfate, 100 mM sodium acetate pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
