4IRR
Crystal Structure of C.elegans Thymidylate Synthase in Complex with dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Collection date | 2011-12-02 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 135.091, 135.091, 155.771 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.200 - 2.480 |
| R-factor | 0.2334 |
| Rwork | 0.232 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.200 |
| High resolution limit [Å] | 2.480 |
| Number of reflections | 57989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.1M Bis-Tris pH 7.2, 0.2M NaAcetate, 15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
