4IRE
Crystal structure of GLIC with mutations at the loop C region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.030, 133.620, 161.430 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 29.857 - 3.190 |
| R-factor | 0.2045 |
| Rwork | 0.204 |
| R-free | 0.24300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4f8h |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.380 |
| High resolution limit [Å] | 3.190 | 9.160 | 3.190 |
| Rmerge | 0.068 | 0.020 | 0.707 |
| Number of reflections | 61417 | 2615 | 9335 |
| <I/σ(I)> | 14.07 | 49.84 | 1.83 |
| Completeness [%] | 97.5 | 94.8 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.9 | 277 | 10-12% PEG 4000, 225 mM ammonium sulfate, 50 mM sodium acetate buffer (pH 3.9 - 4.1), VAPOR DIFFUSION, SITTING DROP, temperature 277K |
