4IP0
X-Ray Structure of the Complex Uridine Phosphorylase from Vibrio cholerae with Phosphate Ion at 1.29 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.671, 71.062, 87.908 |
| Unit cell angles | 69.63, 72.56, 85.73 |
Refinement procedure
| Resolution | 18.934 - 1.294 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.216 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.794 | 18.934 | 1.360 |
| High resolution limit [Å] | 1.294 | 4.090 | 1.290 |
| Rmerge | 0.028 | 0.266 | |
| Total number of observations | 65746 | 127989 | |
| Number of reflections | 327958 | ||
| <I/σ(I)> | 14.2 | 23.2 | 2.3 |
| Completeness [%] | 95.5 | 98.5 | 94.3 |
| Redundancy | 3.8 | 6.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | potassium phosphate, ammonium sulfate, sodium sulfate, pH 7.5, VAPOR DIFFUSION, temperature 291K |
