4IOM

N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with folate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210r
Spacegroup name	H 3 2
Unit cell lengths	160.990, 160.990, 256.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	122.510 - 2.999
R-factor	0.22073
Rwork	0.213
R-free	0.29094
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3QB6
RMSD bond length	0.010
RMSD bond angle	1.498
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.510	3.110
High resolution limit [Å]	2.999	2.999
Rmerge	0.165
Number of reflections	25893
<I/σ(I)>	7.8
Completeness [%]	98.8	100
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	38 to 46% (w/v) saturated ammonium sulfate (AS), 1 mM DTT, and 1 to 3.5% (w/v) PEG 1000 or PEG 1450, in 50 - 75 mM KMB (pH 7.0 - 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K