4IK3

High resolution structure of GCaMP3 at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-02-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	125.993, 46.614, 68.585
Unit cell angles	90.00, 100.09, 90.00

Refinement procedure

Resolution	31.011 - 2.007
R-factor	0.1865
Rwork	0.185
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.966
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.011	2.040
High resolution limit [Å]	2.000	2.000
Number of reflections	25387
Completeness [%]	94.5	68.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	Tris pH 8.5, (NH4)2SO4, 23% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K