4IJX
Crystal structure of human Ap4A hydrolase E58A mutant complexed with DPO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Collection date | 2012-06-21 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.369, 72.369, 133.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.19079 |
| Rwork | 0.189 |
| R-free | 0.23205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u53 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.642 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.157 |
| High resolution limit [Å] | 2.100 | 2.103 |
| Number of reflections | 20214 | |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 285 | 0.1M Tris, 2.0M Ammonium phosphate monobasic, 5mM magnesium chloride, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
